Plasmid

Part:BBa_I713022:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pKD-pBL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2208
    Illegal XbaI site found at 3430
    Illegal PstI site found at 2865
    Illegal PstI site found at 3452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2865
    Illegal PstI site found at 3452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3078
    Illegal BamHI site found at 3464
    Illegal XhoI site found at 2167
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2208
    Illegal XbaI site found at 3430
    Illegal PstI site found at 2865
    Illegal PstI site found at 3452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2208
    Illegal XbaI site found at 3430
    Illegal PstI site found at 2865
    Illegal PstI site found at 3452
    Illegal NgoMIV site found at 2414
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1301
    Illegal SapI site found at 218
    Illegal SapI site found at 2354
    Illegal SapI site found at 2564


Design Notes

The fragments were built by PCR.


Source

pBluescript II SK(-) pKD13

References